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UJI AKTIVITAS ANTIOKSIDAN FRAKSI SPONS LAUT Stylissa flabelliformis MENGGUNAKAN METODE DPPH DAN ABTS SERTA PENETAPAN METABOLIT SEKUNDERNYA

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UJI AKTIVITAS ANTIOKSIDAN FRAKSI SPONS LAUT
Stylissa flabelliformis MENGGUNAKAN METODE DPPH DAN
ABTS SERTA PENETAPAN METABOLIT SEKUNDERNYA
Andhia Rosyantari
ABSTRAK
Stylissa flabelliformis merupakan jenis spons yang banyak tersebar di Indonesia
termasuk di Nusa Tenggara, namun aktivitas biologis fraksi spons laut ini belum
banyak diketahui termasuk aktivitas antioksidannya. Penelitian ini bertujuan
untuk mengetahui aktivitas antioksidan spons laut S. flabelliformis menggunakan
metode DPPH dan ABTS serta menentukan kadar total fenol (TPC) dan kadar
total flavonoid (TFC). Spons laut yang diekstraksi dengan cara maserasi
menggunakan etanol menghasilkan rendemen sebesar 10,71%. Fraksinasi ekstrak
etanol yang dilakukan dengan metode partisi cair-cair menghasilkan rendemen
fraksi air 50,12%, fraksi etil asetat 29,24% dan fraksi n-heksana 20,64%.
Aktivitas antioksidan dengan metode DPPH didapatkan nilai IC50 fraksi air
sebesar 916,26 ppm, fraksi etil asetat sebesar 501,16 ppm dan fraksi n-heksana
sebesar 3780,23 ppm, sedangkan dengan metode ABTS didapatkan nilai IC50
fraksi air sebesar1257,76 ppm, fraksi etil asetat sebesar 439,26 ppm, dan fraksi nheksana sebesar 2054,06 ppm. Berdasarkan hasil uji beda antara nilai IC50 DPPH
dan ABTS menggunakan uji Mann-Whitney diperoleh nilai signifikansi (p value =
0,932) yang berarti bahwa data tidak berbeda signifikan dan kedua metode
menghasilkan informasi yang sepadan. TPC ekstrak etanol sebesar 0,466
mgGAE/g, fraksi air sebesar 0,548 mgGAE/g, fraksi etil asetat sebesar 0,619
mgGAE/g dan fraksi n-heksana sebesar 0,066 mgGAE/g. TFC ekstrak etanol
sebesar 1,854 mgQE/g, fraksi air sebesar 1,896 mgQE/g, fraksi etil asetat 9,344
mgQE/g dan fraksi n-heksana sebesar 0,062 mgQE/g. Hasil uji korelasi antara
TPC, TFC dengan nilai IC50 (DPPH dan ABTS) menunjukkan nilai p value
berturut-turut adalah 0,997 dan 0,990 yang berarti bahwa kadar total fenol dan
total flavonoid spons laut berkorelasi positif dengan aktivitas antioksidannya.
Kata kunci: Stylissa flabelliformis, antioksidan, kadar total fenol, kadar total
flavonoid.ix
ANTIOXIDANT ACTIVITY ASSAY OF MARINE SPONGE
Stylissa flabelliformis FRACTION USING DPPH AND ABTS
METHODS AND MEASUREMENT OF ITS SECONDARY
METABOLITES
Andhia Rosyantari
ABSTRACT
Stylissa flabelliformis is a type of sponge that is widely distributed in
Indonesia, especially on Sulawesi, Bali and Nusa Tenggara islands, meanwhile
the biological activity of this marine sponge has not been reported, including its
antioxidant activity. This study determined the antioxidant activity of the marine
sponge S. flabelliformis using the DPPH and ABTS method and also its total
phenol content (TPC) and total flavonoid content (TFC). The marine sponge was
extracted by maceration and the extraction yeild was 10.71%. The fractionation
was carried out by the liquid-liquid partition method. The yield of the water
fraction was 50.12%, the ethyl acetate fraction was 29.24% and the n-hexane was
20.64%. The assessment of the IC50 value was carried out with DPPH method and
ABTS method. Using the DPPH method, the IC50 of water fraction was 916.26
ppm, the ethyl acetate fraction was 501.16 ppm and the n-hexane was 3780.23
ppm. As for the ABTS method, the IC50 value of water fraction was 1257.76 ppm,
the ethyl acetate fraction was 439.26 ppm, and the n-hexane was 2054.06 ppm.
Based on the results of the statistics test between the IC50 of DPPH and ABTS
values using Mann-Whitney, there was no significant difference between the two
methods (p value = 932), which means that the data from both methods was not
different. The total phenol content of the ethanol extract was 0,466 mgGAE/g, the
water fraction was 0,548 mgGAE/g, the ethyl acetate fraction was 0,619
mgGAE/g and the n-hexane was 0,066 mgGAE/g. The total flavonoid content of
the ethanol extract was 1,854 mgQE/g, the water fraction was 1,896 mgQE/g, the
ethyl acetate fraction was 9,344 mgQE/g and the n-hexane was 0,062 mgQE/g.
The results of the correlation test between TPC, TFC with IC50 (DPPH and
ABTS) values showed p value = 0.997 and p value = 0.990, respectively, which
means that the levels of total phenol and total flavonoids in the marine sponge are
positively correlated with the antioxidant activity.
Keywords: Stylissa flabelliformis, antioxidant, total phenolic content, total
flavonoid content.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

157

Penerbit

Mataram : Farmasi FK Unram., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

DTS 610 And u F.2022

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

SKRIPSI

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

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