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PENENTUAN KADAR FLAVONOID TOTAL EKSTRAK DAN FRAKSI-FRAKSI DAUN MANGROVE (Avicennia marina)

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ABSTRAK
Avicennia marina (tumbuhan mangrove) berasal dari famili Avicenniaceae.
Daunnya memiliki aktivitas antioksidan, antivirus, dan antibakteri yang disebabkan
oleh kandungan metabolit sekunder pada daun mangrove berupa senyawa
flavonoid. Penelitian terkait kandungan flavonoid total pada ekstrak etanol 96%
maupun fraksi air, etil asetat, dan n-heksana daun A. marina belum pernah
dilaporkan. Tujuan penelitian ini untuk menentukan kadar flavonoid total pada
ekstrak dan fraksi daun mangrove A. marina dengan metode spektrofotometri UVVis. Ekstraksi dilakukan menggunakan pelarut etanol 96% dengan metode sonikasi,
kemudian dilakukan fraksinasi dengan pelarut n-heksana, etil asetat, dan air.
Ekstrak dan fraksi-fraksi yang diperoleh diidentifikasi metabolit sekundernya
dengan uji tabung dan kromatografi lapis tipis kemudian ditentukan kadar flavonoid
totalnya dengan metode kolorimetri. Ekstrak dan fraksi-fraksi daun mangrove
mengandung metabolit sekunder flavonoid, fenolik, saponin, steroid, triterpenoid,
dan alkaloid. Hasil kromatografi lapis tipis menegaskan hasil uji tabung bahwa
ekstrak dan fraksi-fraksi daun mangrove mengandung senyawa flavonoid. Kadar
flavonoid total pada ekstrak etanol 96%, fraksi air, fraksi etil asetat, dan fraksi nheksana daun mangrove A. marina berturut-turut sebesar 19,37; 3,07; 11,30; dan
56,62 mg EK/gram sampel.
Kata kunci : Daun mangrove (Avicennia marina), kadar flavonoid total,
spektrofotometri UV-Visviii
ABSTRACT
Avicennia marina (mangrove plants) originated from the Avicenniaceae
family. The leaves have antioxidant, antiviral and antibacterial activity caused by
the content of secondary metabolites in mangrove leaves in the form of flavonoid
compounds. Research on the total flavonoid content of 96% ethanol extract and the
water, ethyl acetate, and n-hexane fractions of A. marina leaves has never been
reported. The purpose of this study was to determine the total flavonoid content in
extracts and fractions of A. marina mangrove leaves by UV-Vis spectrophotometry
method. Extraction was carried out using 96% ethanol by sonication method, then
fractionation was carried out using n-hexane, ethyl acetate and water as solvents.
The extracts and fractions obtained for their secondary metabolites were identified
by test tube and thin layer chromatography and then the total flavonoid content was
determined by the colorimetric method. Mangrove leaf extracts and fractions
contain secondary metabolites of flavonoids, phenolics, saponins, steroids,
triterpenoids, and alkaloids. The results of thin layer chromatography confirmed the
results of the tube test that mangrove leaf extracts and fractions contained flavonoid
compounds. Total flavonoid content in 96% ethanol extract, water fraction, ethyl
acetate fraction, and n-hexane fraction of A. marina mangrove leaves were 19.37;
3.07; 11.30; and 56,62 mg EQ/gram sample.
Keyword : Mangrove leaves (Avicennia marina), total flavonoid content,
spectrophotometry UV-Vis

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

213

Penerbit

Mataram : Farmasi FK Unram., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

DTS 610 Zul p F.2023

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

SKRIPSI

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

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