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PENENTUAN KADAR FENOLIK TOTAL EKSTRAK DAN FRAKSI-FRAKSI DAUN KEPUNDUNG (Baccaurea racemosa)

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ABSTRAK
Baccaurea racemosa merupakan salah satu tumbuhan berkhasiat obat yang
secara empiris bagian daunnya dimanfaatkan untuk mengobati diare dan
melancarkan haid. Aktivitas tersebut diperoleh karena daun B. racemosa
mengandung senyawa metabolit seperti flavonoid dan fenolik. Beberapa penelitian
menunjukkan bahwa senyawa fenolik yang terkandung pada ekstrak daun B.
racemosa memiliki aktivitas antioksidan potensial. Namun, penelitian mengenai
kadar senyawa fenolik total yang terkandung dalam ekstrak dan fraksi daun
kepundung (B. racemosa) b elum memadai. Oleh karena itu, penelitian ini
dilakukan untuk menetapkan kadar senyawa fenolik total yang terkandung dalam
ekstrak dan fraksi-fraksi daun kepundung (B. racemosa). Ekstraksi senyawa
dilakukan menggunakan metode sonikasi dengan pelarut etanol 96% dan
dilanjutkan partisi cair-cair dengan pelarut n-heksan, etil asetat, dan akuades.
Ekstrak dan fraksi-fraksi yang diperoleh dianalisis profil metabolitnya dengan uji
tabung dan kadar fenolik total dengan spektrofotometri UV-Vis. Perolehan kadar
fenolik total kemudian dianalisis statistik dengan metode one-way analysis of
variance (ANOVA) dan dilanjutkan dengan uji Tukey. Hasil uji tabung
menunjukkan ekstrak dan fraksi-fraksi daun kepundung mengandung senyawa
fenolik, flavonoid, steroid, dan saponin. Kadar fenolik total pada ekstrak etanol
96%, fraksi n-heksan, etil asetat, dan akuades secara berturut-turut sebesar 419,153
? 1,80; 115,589 ? 1,68; 244,359; dan 334,512 ? 1,29 mg ekivalen asam galat
(EAG)/gram ekstrak dan fraksi. Hasil uji statistik menunjukkan seluruh sampel
memiliki kadar fenolik total yang berbeda signifikan.
Kata Kunci: B. racemosa, kepundung, antioksidan, senyawa fenolik, ekstrak,
fraksi.viii
ABSTRACT
Baccaurea racemosa is one of the medicinal plants which leaves are
empirically used to treat diarrhea and launch menstruation. This activity was
obtained because B. racemosa leaves contain metabolite compounds such as
flavonoids and phenolics. Several studies have shown that the phenolic compounds
contained in B. racemosa leaf extract have potential antioxidant activity. However,
research on the levels of total phenolic compounds contained in the extract and
fractions of kepundung leaves (B. racemosa) has not been sufficient. Therefore, this
research was conducted to determine the levels of total phenolic compounds
contained in the extracts and fractions of kepundung leaves (B. racemosa).
Compound extraction was carried out using the sonication method with 96%
ethanol solvent and followed by liquid-liquid partitioning with n-hexane, ethyl
acetate and distilled water. The extracts and fractions obtained were analyzed for
their metabolite profiles by test tube and total phenolic content by UV-Vis
spectrophotometry. The obtained total phenolic content was then analyzed
statistically using the one-way analysis of variance (ANOVA) method and followed
by the Tukey test. Test-tube results showed that extracts and fractions of kepundung
leaves contained phenolic compounds, flavonoids, steroids and saponins. The total
phenolic content in the 96% ethanol extract, n-hexane fraction, ethyl acetate, and
distilled water respectively were 419.153 ? 1.80; 115.589 ? 1.68; 244,359; and
334.512 ? 1.29 mg gallic acid equivalent (GAE)/gram extract and fraction. The
statistical test results showed that all samples had significantly different phenolic
levels.
Keywords: B. racemosa, kepundung, antioxidants, phenolic compounds, extracts,
fractions

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Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

233

Penerbit

Mataram : Farmasi FK Unram., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

DTS 610 Din p F.2023

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

-

Subjek

SKRIPSI

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

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