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FORMULASI LIPOSOM EKSTRAK ETANOL DAUN MANGROVE (Sonneratia alba) DAN UJI AKTIVITAS ANTIRADIKAL BEBAS

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ABSTRAK
Peran kulit sebagai protektor utama terhadap paparan radiasi ultraviolet
menyebabkannya rentan mengalami kerusakan. Ekstrak etanol daun mangrove
Sonneratia alba dapat diformulasi menjadi sediaan topikal sebagai agen proteksi
terhadap ultraviolet, akan tetapi terdapat kendala terkait stabilitas dan penetrasinya.
Liposom merupakan sistem penghantaran obat dengan struktur menyerupai lapisan
kulit sehingga dapat menjadi solusi terkait permasalahan tersebut. Penelitian ini
bertujuan untuk mengetahui formula dan karakteristik liposom ekstrak etanol
S.alba serta aktivitas penghambatan radikal bebas liposom. Daun mangrove
diekstrak dengan metode sonikasi, hasil ekstraksi dilakukan uji metabolit sekunder
menggunakan metode tabung dan KLT. Formulasi liposom dilakukan dengan
metode hidrasi lapis tipis, kemudian dilakukan karakterisasi ukuran partikel, indeks
polidispersi, dan efisiensi penjerapan, serta uji penghambatan radikal bebas
liposom dengan metode DPPH. Hasil karakterisasi dianalisis dengan statistika
deskriptif, sementara perbandingan persentase inhibisi ekstrak dan liposom
dianalisis secara statistik paired t-test (p>0,05). Hasil uji tabung dan KLT
menujukkan ekstrak S.alba teridentifikasi mengandung fenolik dan flavonoid.
Liposom dengan variasi bobot 50;100;dan 200 mg menghasilkan efisiensi
penjerapan berturut-turut 95,4884? 0,5207%; 94,8419?0,4092%; dan
88,7686?0,3376. Ukuran partikel liposom yang terbentuk 2018?228,30 nm dan
indeks polidispersi 0,654?0,056. Ekstrak etanol daun mangrove dengan konsentrasi
8 ppm mampu menghambat 50,40% radikal DPPH . Sementara dengan konsentrasi
yang sama, liposom menghasilkan penghambatan 39,59 ? 3,38%. Hasil ini
mendukung pengembangan ekstrak S.alba dalam sistem liposom sebagai
penghantaran obat untuk proteksi dari sinar ultraviolet.
Kata Kunci : Sonneratia alba, Liposom, Antiradikal Bebas DPPH, Hidrasi Lapis
Tipisix
ABSTRACT
The skin's role as the main protector against exposure to ultraviolet radiation
makes it susceptible to damage. Ethanol extract of Sonneratia alba mangrove
leaves can be formulated into topical preparations as a protective agent against
ultraviolet rays, however there are obstacles regarding its stability and penetration.
Liposomes, a drug delivery system with a structure resembling a skin layer, can be
a solution to this problem. This study aims to determine the formula and
characteristics of S.alba ethanol extract liposomes as well as the free radical
inhibitory activity of the liposomes. Mangrove leaves were extracted using the
sonication method, the extraction results were tested for secondary metabolites
using the tube and TLC methods. Liposome formulation was carried out using the
thin layer hydration method, then particle size, polydispersity index and adsorption
efficiency were characterized. The free radical inhibitory activity of liposomes was
tested via the DPPH method with ascorbic acid standards using a UV-Vis
spectrophotometry instrument. The characterization results were analyzed using
descriptive statistics and the IC50 value was calculated from the percentage of
liposome inhibition. The percent inhibition value of mangrove leaf ethanol extract
before and after being formulated into liposomes was analyzed statistically using
the paired t test method with a confidence level of p>0.05. The results of the tube
and TLC tests showed that the S. alba extract was identified as containing phenolics
and flavonoids. Liposomes with varying weights of 50; 100; and 200 mg produced
entrapment efficiencies of 95.4884 ? 0.5207%; 94.8419?0.4092%; and 88.7686 ?
0.3376. The particle size of the liposomes formed was 2018 ? 228.30 nm and the
polydispersion index was 0.654 ? 0.056. Mangrove leaf ethanol extract with a
concentration of 8 ppm is able to inhibit 50.40% of DPPH radicals. Meanwhile,
with the same concentration, liposomes produced an inhibition of 39.59 ? 3.38%.
These results support the development of S.alba extract in a liposome system as
drug delivery for protection from ultraviolet light.
Keyword : Sonneratia alba, Liposome, Free Radical, DPPH, Thin Film Hydration

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

238

Penerbit

Mataram : Farmasi FK Unram., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

DTS 610 Dhi f F.2023

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

SKRIPSI

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

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