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PENGARUH PERBEDAAN PELARUT PADA PROFIL METABOLIT EKSTRAK RUMPUT LAUT Halimeda sp. dan Turbinaria sp

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ABSTRAK
Halimeda sp. dan Turbinaria sp. merupakan sumber penghasil berbagai
macam senyawa metabolit yang memiliki aktivitas farmakologis maupun biologis.
Keanekaragaman metabolit dapat dipengaruhi oleh beberapa faktor seperti faktor
lingkungan maupun penggunaan pelarut pada proses ekstraksi. Penelitian ini
bertujuan untuk mengetahui perbedaan profil metabolit serta senyawa mayor pada
ekstrak n-heksana dan n-heksana:metanol rumput laut Halimeda sp. dan
Turbinaria sp. di Perairan Pantai Seriwe, Kabupaten Lombok Timur, NTB.
Rumput laut Halimeda sp. dan Turbinaria sp. diekstraksi menggunakan metode
maserasi dan sokletasi. Uji fitokimia dilakukan secara kualitatif menggunakan
metode tabung dan KLT, sedangkan uji secara kuantitatif dilakukan dengan
menggunakan GC-MS, serta penetapan kadar total fenol dan total flavonoid
dengan metode spektrometri. Hasil penelitian ini menunjukkan bahwa ekstrak
Halimeda sp. dan Turbinaria sp. mengandung senyawa flavonoid, alkaloid, fenol,
terpenoid, steroid, tanin dan lipid triasilgliserol. Hasil analisis dengan
menggunakan GC-MS menandakan adanya senyawa mayor berupa asam lemak
serta pada penetapan kadar fenol dan flavonoid masing-masing sampel memiliki
perbedaan kadar secara signifikan. Kadar fenol Halimeda sp. dan Turbinaria sp.
tertinggi berturut-turut sebesar 11,31 mgGAE/g dan 7,74 mgGAE/g, sedangkan
kadar flavonoid Halimeda sp. dan Turbinaria sp. tertinggi berturut-turut sebesar
20,48 mgQE/g dan 19,67 mgQE/g.
Kata kunci: Fitokimia, GC-MS, Halimeda sp., Kadar Fenol dan Flavonoid,
Turbinaria sp.vii
ABSTRACT
Halimeda sp. and Turbinaria sp. are sources of various metabolites with
pharmacological and biological activities. The diversity of these metabolites can
be influenced by several factors such as environmental conditions and the solvents
used in the extraction process. This study aims to identify the differences in
metabolite profiles and major compounds in n-hexane and n-hexane:methanol
extracts of Halimeda sp. and Turbinaria sp. from the coastal waters of Seriwe
Beach, East Lombok Regency, NTB. Halimeda sp. and Turbinaria sp. were
extracted using maceration and Soxhlet extraction methods. Phytochemical testing
was conducted qualitatively using tube and TLC methods, while quantitative
testing was performed using GC-MS, and the determination of total phenolic and
flavonoid content was conducted using spectrometry methods. The results showed
that the extracts of Halimeda sp. and Turbinaria sp. contained flavonoids,
alkaloids, phenols, terpenoids, steroids, tannins, and triacylglycerol lipids.
Analysis using GC-MS indicated the presence of major compounds in the form of
fatty acids, and the determination of phenolic and flavonoid content in each
sample revealed significant differences in their levels. The highest phenolic
content in Halimeda sp. and Turbinaria sp. was 11.31 mgGAE/g and 7.74
mgGAE/g, respectively, while the highest flavonoid content in Halimeda sp. and
Turbinaria sp. was 20.48 mgQE/g and 19.67 mgQE/g, respectively.
Keywords: Phytochemistry, GC-MS, Halimeda sp., Phenolic and Flavonoid
Content, Turbinaria sp

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

277

Penerbit

Mataram : Farmasi FKIK Unram., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

DTS 610 Erv p F.2024

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

SKRIPSI 2024

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

Lampiran Berkas

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